Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia

The black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene morphological characteristics colonies, sporangia, chlamydospores hyphae were used to identify isolates. Races identified according reaction induced each isolate on differential varieties Hicks, L8, KY 14 x L8 NC 1071. As results, 71 isolates classified races. Colonies white color, cottony fluffy texture with smooth, non-swollen hyphae; spherical papillae an average 1.26 μm non-papillated intercalary medium size 1.02 that typical nicotianae. A species-specific PCR-amplified band 389 bp was detected all tested. presence races 0, 1 3 determined Colombian departments Huila Santander. To best our knowledge, this is first report physiological Colombia. Results relevance for management breeding.